[Histonet] Cutting blocks for mouse tissue troubleshooting

[Blanca Lopez]

Hello Histonets!
I  need to find out a better way to cut the whole pancreas mouse tissue without wrinkles, Does anybody has suggestion. I am having trouble to obtain a nice section without wrinkles. It is pretty big piece and crawl in on my embedding, is hard to stretch out without breaking. I cannot use ammonia water because they use the unstained for research and I don't want get discrepancies in any other stains.  Suggestions???
thanks

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: Blanca.Lopez at utsouthwestern.edu


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