[Histonet] ER/PR Uneven Staining

[Cartun, Richard]

Can you provide more details about the uneven immunoreactivity?  Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, November 29, 2016 4:09 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] ER/PR Uneven Staining

I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with  needle core biopsies has always been more than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR antibodies on the BOND.



Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com


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