[Histonet] ER/PR Uneven Staining

Joanne Clark jclark at pcnm.com
Tue Nov 29 15:09:17 CST 2016

I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with  needle core biopsies has always been more than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR antibodies on the BOND.

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284


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