[Histonet] ER/PR Uneven Staining

[Joanne Clark]

It's rather strange.  There are positive tumor cells throughout the needle core, but the bottom third is staining very strongly all across the core, then there is a small portion where the staining is there but not as strongly as the section below it, then another segment above this where the staining is strong again.  The strong and weak staining is across the entire core of the tissue, not just at the edges.

-----Original Message-----
From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org] 
Sent: Tuesday, November 29, 2016 3:12 PM
To: Joanne Clark
Cc: histonet at lists.utsouthwestern.edu
Subject: RE: ER/PR Uneven Staining

Can you provide more details about the uneven immunoreactivity?  Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, November 29, 2016 4:09 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] ER/PR Uneven Staining

I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with  needle core biopsies has always been more than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR antibodies on the BOND.



Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com


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