[Histonet] Florida State Licensure
Marsha Price
mprice63 at live.com
Wed May 11 10:59:17 CDT 2016
Can someone provide the link for the application to obtain HT Florida State Licensure
Sent from my iPhone
> On May 10, 2016, at 12:00 PM, <histonet-request at lists.utsouthwestern.edu> <histonet-request at lists.utsouthwestern.edu> wrote:
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> Today's Topics:
>
> 1. IHC Wet Workshop announcement (Ihc Workshop)
> 2. Re: PAS Stain (Seeley, Heather)
> 3. Re: Tracking surgical specimens (Jamieson Anderson)
> 4. Tips on staining PLA2R assay on Leica Bond (Eddie Martin)
> 5. Re: PAS Stain (victor_tobias at comcast.net)
> 6. P16 (Charles Riley)
> 7. Cytology/Histology Staining Question (Mullen, Mary)
> 8. Re: Cytology/Histology Staining Question (Rene J Buesa)
> 9. ASCP Certified Pathologist Assistant Job Opening- (Melissa Owens)
> 10. Melanin Bleach (Heckford, Karen - SMMC-SF)
> 11. Re: Melanin Bleach (Jeffrey Robinson)
> 12. Re: Melanin Bleach (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 9 May 2016 10:16:39 -0700
> From: Ihc Workshop <ihcworkshop at gmail.com>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] IHC Wet Workshop announcement
> Message-ID: <3FAC1E78-7C1B-4CBC-9A99-FDA3F2BAF430 at gmail.com>
> Content-Type: text/plain; charset=us-ascii
>
> June 23 & 24, 2016. San Francisco Bay Area
> This IHC lab course is aimed at hands-on training of attendees in all aspects of IHC staining of human, mouse and animal tissues with varied antibodies. This is a small group workshop and is taught in a lab.....Lots of troubleshooting, only few spots left.
> To get more detail contact. Maria at: ihcworkshop at gmail.com
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 9 May 2016 17:35:02 +0000
> From: "Seeley, Heather" <Heather.Seeley at tenethealth.com>
> To: Bob Richmond <rsrichmond at gmail.com>,
> "Histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] PAS Stain
> Message-ID:
> <9DFE334E776E734D9D231EF60CCE93C57E26DB05 at TENHDCTHMB10-31.tenethealth.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> We have one girl that always spits on our slide! :) works great!
>
> HEATHER SEELEY, HT(ASCP)
> Histotech
> 803-985-4676 OFFICE
> 803-327-7598 FAX
>
>
> ________________________________________
> From: Bob Richmond [rsrichmond at gmail.com]
> Sent: Thursday, May 05, 2016 2:10 PM
> To: Histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS Stain
>
> Amylase (diastase) for the PAS stain queries:
>
> Whatever happened to spitting on the slide (30 min at room temperature)?
> John Kiernan advises "thinking of lemons and drooling into a small beaker"
> though I'd advise chewing on a rubber band for a few seconds.
>
> He notes that alpha amylase is preferred. I'd go with the cheapest one in
> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma
> offers a heat-stable alpha amylase.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 9 May 2016 17:41:09 +0000 (UTC)
> From: Jamieson Anderson <jamiesonanderson at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Tracking surgical specimens
> Message-ID:
> <537327FDD6BA9F6C.0B786CD3-7FD7-4574-B1DC-3D754EACB582 at mail.outlook.com>
>
> Content-Type: text/plain; charset=us-ascii
>
> Hi Lynne,
> In our lab we have a log book at Accessioning that each physician/nurse/porter/MOA signs when dropping off specimens. We also encourage them to drop off a QC sheet with a patient label for each specimen that is dropped off. We check these to ensure we have received each specimen, and we date/time stamp them and keep them on record in case we need to refer back to them in the future (ie. In case a clinician claims they dropped off a specimen we can prove they did not).
> Jamieson AndersonTechnical Coordinator - Anatomic PathologySt. Paul's HospitalLower Mainland Pathology & Laboratory Medicine
>
> _____________________________
>
> Date: Mon, 9 May 2016 14:49:56 +0000
> From: "Bell, Lynne" <Lynne.Bell at cvmc.org>
> To: "Histonet (histonet at lists.utsouthwestern.edu)"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Tracking surgical specimens brought to the lab
> Message-ID:
> <EF83D5D097374D4497AF4622C35BE29301862AA3C1 at CVMC-EMAILMBX1.CVMC.ORG>
> Content-Type: text/plain; charset="us-ascii"
>
> For those of you who have specimens brought to your lab: do you have the person dropping off the specimen(s) initial a manifest to keep track of specimens actually dropped off. Case in point - a physician's office says they dropped off a specimen and we have no record of it being accessioned. I would like to track specimens dropped off and I am not sure how to accomplish this.
>
> I appreciate any feedback!
>
> Thanks,
>
>
> Lynne Bell, HT (ASCP)
> Histology Team Leader
> Central Vermont Medical Center
> 130 Fisher Road
> Berlin, VT 05641
> (802)371-4923
>
>
>
>
>
>
> ------------------------------
>
> Subject: Digest Footer
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> ------------------------------
>
> End of Histonet Digest, Vol 150, Issue 12
> *****************************************
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Mon, 9 May 2016 12:51:46 -0500
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Tips on staining PLA2R assay on Leica Bond
> Message-ID: <16EDC8EC-EDAB-4F63-A3BD-BEC24EAB0BE3 at gmail.com>
> Content-Type: text/plain; charset=utf-8
>
> I don?t do staining for this antibody, but have experience with IMF on frozen tissue and FFPE and have worked with the Leica BOND max and BOND-3. If you would prefer IHC over IMF, you can search for a human anti-mouse, or human anti-rabbit antibody. Abcam provides a polyclonal whole serum antibody that works on FFPE. I grabbed this from their website:
> Anti-PLA2R antibody (ab80054)
>
> Since Abcam?s PLA2R antibody is a rabbit anti-human, you would need to create a modified DAB or modified RED protocol that doesn?t include the secondary antibody / linker step in your protocol prior to dispensing polymer. The rest of your protocol can remain as it is.
>
> As you are using a Leica BOND, both ways are actually pretty easy to set up, either indirect IMF or using Leica?s DAB kit for IHC. You would just need to play around with retrieval times with both Citrate and EDTA to get the optimum staining pattern. Additional ancillaries may be added when necessary should you have any background stain that is hard to get out.
>
> Best Regards,
>
> Eddie Martin, HT(ASCP), QIHC
> 954-826-9403
> Edmartin26 at gmail.com
>
> ------------------------------
>
> Message: 5
> Date: Mon, 9 May 2016 22:39:38 +0000 (UTC)
> From: victor_tobias at comcast.net
> To: Heather Seeley <Heather.Seeley at tenethealth.com>,
> "Histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>, Bob Richmond
> <rsrichmond at gmail.com>
> Subject: Re: [Histonet] PAS Stain
> Message-ID:
> <1237907839.22424188.1462833578398.JavaMail.zimbra at comcast.net>
> Content-Type: text/plain; charset="utf-8"
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 10 May 2016 05:47:18 -0400
> From: Charles Riley <criley at dpspa.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] P16
> Message-ID:
> <CAAQhB13E__iaj-X1FGn7zKrE1_BmkrrgFi2ZFtsgkrzrravT0w at mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Has anyone found a way to do p16 staining without purchasing anything from
> Ventana?
>
> My company wants to do P16 but refuses to by any ventana products and I
> have explained it is a waste of money to keep testing the antibodies from
> all the other companies.
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 10 May 2016 14:54:55 +0000
> From: "Mullen, Mary" <mullenmk at mail.magee.edu>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Cytology/Histology Staining Question
> Message-ID:
> <374DC72E6B29D44086F8FF3289351B2508823897 at MSXMBXNSPRD39.acct.upmchs.net>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello all,
>
>
>
> I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).
>
>
>
> What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues?
>
>
>
> We only run non-gyn cytology, all gyn cytology is sent out.
>
>
>
>
>
>
>
> Thanks,
>
>
>
> Mary K. Mullen, HTL(ASCP)CM
> Histotechnologist
> UPMC Northwest
> Seneca, PA
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 10 May 2016 15:07:04 +0000 (UTC)
> From: Rene J Buesa <rjbuesa at yahoo.com>
> To: "Mullen, Mary" <mullenmk at mail.magee.edu>,
> "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Cytology/Histology Staining Question
> Message-ID:
> <1252522947.1519943.1462892824143.JavaMail.yahoo at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> I would be concerned with potential cross-contamination. In my lab we had 2 staining instruments, one for cytology and other for histology.Ren?
>
> On Tuesday, May 10, 2016 10:59 AM, "Mullen, Mary via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
>
>
> Hello all,
>
>
>
> I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).
>
>
>
> What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues?
>
>
>
> We only run non-gyn cytology, all gyn cytology is sent out.
>
>
>
>
>
>
>
> Thanks,
>
>
>
> Mary K. Mullen, HTL(ASCP)CM
> Histotechnologist
> UPMC Northwest
> Seneca, PA
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 10 May 2016 15:44:21 +0000
> From: Melissa Owens <melissa at alliedsearchpartners.com>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] ASCP Certified Pathologist Assistant Job Opening-
> Message-ID: <D3577A10.215B8%melissa at alliedsearchpartners.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello,
>
> I have a Full Time/Permanent job opening for an ASCP certified Pathologist Assistant in Ohio. Please contact me for details. Have a great day!
>
> Melissa Owens
> President, Laboratory Staffing
> Allied Search Partners
>
>
> T: 888.388.7571 ext. 102
>
> F: 888.388.7572
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 10 May 2016 08:44:33 -0700
> From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Melanin Bleach
> Message-ID:
> <D329219D3B967447A9E3ED3242117E3216826A1496 at PHX-MSG-007-N1.chw.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
> I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20.
>
> Thanks,
>
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org
> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 10 May 2016 16:33:26 +0000
> From: Jeffrey Robinson <JRobinson at pathology-associates.com>
> To: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
> Cc: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Melanin Bleach
> Message-ID:
> <204A03EB5A7F0A4BB1EEDD52A963829C90A16313 at PAEXCH1.PathologyAssociates.local>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Karen- I have also had problems getting tissue being stained for IHC markers to adhere to the slide. The Potassium permanganate is really harse on the tissue (if you can get the sections to stay on). Here is a trick I picked up at an NSH lecture that I have used successfully several times.
>
> Run your IHC stains as usual. Rinse well in water. Do not counterstain with hematoxylin. Stain with DiffQuik II for 30 seconds. Rinse in water for a short period of time. Differentiate with 10 dips each in 2 changes of 95% ETOH and 2 changes of 100% ETOH and then clear in xylene and coverslip.
> Results: melanin pigment will turn green. Brown DAB stain will be unaffected. Other tissue elements will be stained a medium blue color.
> This method will also work for other Special Stains but I have not attempted to modify this method for use on "Red" stains.
>
> Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
>
> -----Original Message-----
> From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Tuesday, May 10, 2016 8:45 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Melanin Bleach
>
> Good Morning,
> I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20.
>
> Thanks,
>
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org
> Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
>
>
>
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>
> This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you.
>
>
>
> ------------------------------
>
> Message: 12
> Date: Tue, 10 May 2016 16:36:54 +0000 (UTC)
> From: Rene J Buesa <rjbuesa at yahoo.com>
> To: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>,
> "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Melanin Bleach
> Message-ID:
> <930716214.1612034.1462898214077.JavaMail.yahoo at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
>
> You are right. Bleaching is a "rough" procedure for the "survival" of sections and if on top of that you left the section overnight in DiH2O that is a recipe for disaster, as the one you experienced. Try to do the whole procedure during the same day.Additionally it seems to me that 6h in potassium permanganate is too much, you should check the condition of the sections every hour trying to minimize KMnO4?action?the least time possible.Ren?
>
> On Tuesday, May 10, 2016 12:10 PM, "Heckford, Karen - SMMC-SF via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
>
>
> Good Morning,
> I need some help.? Yesterday I bleached some heavily pigmented tissue.? I have to run some IHC's on them.? I bought the bleaching kit from American Master Tech.? I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid.? I had to let them set overnight because I could not get another IHC run in that day.? It looks like the tissue fell off during decloaking.? ? I used Apex slides.? I rarely ever have to bleach anything here.? So I am not sure if I did this correctly.? I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20.
>
> Thanks,
>
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckford at dignityhealth.org
> ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you."
>
>
>
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>
>
>
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