[Histonet] plastic to paraffin

[Morken, Timothy]

Tamara, Yes, it is possible, and some do immuno on plastic sections with this method, but it's a lot of work and the issue arises of whether you have validated your stains to work when this method is used. And do you have controls treated the same way? In the clinical diagnostic environment, in  purely practical terms, it is not worthwhile. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-----Original Message-----
From: Tamara Howard via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, May 11, 2016 1:34 PM
To: kenneth.metzger at aruplab.com
Cc: histonet at lists.utsouthwestern.edu
Subject: [Histonet] plastic to paraffin

Kenneth -


You can do this, but it won't be pretty - if you trim as much of the plastic away as you can, you can etch the resin away with sodium methoxide (or ethoxide):


http://www.ncbi.nlm.nih.gov/pubmed/357729

[http://www.ncbi.nlm.nih.gov/coreutils/img/pubmed256blue.png]<http://www.ncbi.nlm.nih.gov/pubmed/357729>

A method for the removal of epoxy resins from tissue in preparation for scanning electron microscopy. - PubMed - NCBI <http://www.ncbi.nlm.nih.gov/pubmed/357729>
www.ncbi.nlm.nih.gov
J Microsc. 1978 May;113(1):95-9.


Good luck!


Tamara


...................................................
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM

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