[Histonet] Histo/Cyto staining

[Terri Braud]

We run both stains on our Sakura Prisma, but they share no common reagents except for the final Xylene unload station.  We do use the combination CytoKwik stain, which combines the OG6 and EA50 steps, to reduce the number of steps in our Cytology stain procedure. We only run non-gyn, too.  It is sooooo much easier!

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874



Message: 7
Date: Tue, 10 May 2016 14:54:55 +0000
From: "Mullen, Mary" <mullenmk at mail.magee.edu>
Subject: [Histonet] Cytology/Histology Staining Question
Hello all,
I work in a small, low volume community hospital and was recently asked by a coworker why we do not just run both our cytology and histology slides on the same automated stainer (with their respective protocols).
What I am wanting to know is if there is anyone currently running both staining protocols on a single automated stainer using common alcohols/xylenes/water? What are the pros/cons? Has there been any cross-contamination issues.
We only run non-gyn cytology, all gyn cytology is sent out.
Thanks,
Mary K. Mullen, HTL(ASCP)CM
Histotechnologist
UPMC Northwest
Seneca, PA