[Histonet] PAS Stain

[Gudrun Lang]

As far as I remember the incubation temperature is at roomtemperature. 60°C
would rather denature the native enzyme than increase activity.

Look at the optimal working temp of the reagens you have bought.

regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 04. Mai 2016 22:03
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] PAS Stain

Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS
diastase method.  We have been digesting the tissue in 0.5% diastase of malt
in a 60 degree oven for 30 minutes, but do not see the glycogen being
digested out.  I have tried alpha amylase and beta amylase also without any
luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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