[Histonet] PAS Stain

[Joanne Clark]

Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method.  We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out.  I have tried alpha amylase and beta amylase also without any luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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