[Histonet] PAS Stain

[Manfre, Philip]

We switched to alpha amylase since the diastase we were ordering was not working.  We use a 2% solution of alpha amylase for 40 minutes at room temperature and it has been working fine.

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre at merck.com



-----Original Message-----
From: Joanne Clark via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, May 04, 2016 4:03 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] PAS Stain

Hi Histonetters, I'm hoping someone can help me troubleshoot our PAS diastase method.  We have been digesting the tissue in 0.5% diastase of malt in a 60 degree oven for 30 minutes, but do not see the glycogen being digested out.  I have tried alpha amylase and beta amylase also without any luck.  Does anyone have any suggestions to get the digestion to work

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284

pcnm.com




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