[Histonet] CD34 for primate tissue

[Croix, Denise]

Hi Cathy,

When I was doing work with NHP tissue (both flow cytometry and IHC) there
were several sources that I utilized for info on antibodies that were
cross-reactivity with various NHP species.  The NIH hosts a NHP reagent
resource website (nhpreagents.org) that shows which commercially available
antibodies cross react with non-human primate tissues.  They note that
there are discrepancies reported about cross-reactivity with the QBEND10
antibody for  rhesus and possibly cynos.  You will need to still check with
the vendors to confirm if the antibody has been tested in FFPE specimens as
that is not indicated on the site but it should give you a starting point
to work from.

I've had personal experience with cyno & rhesus T cells where individual
monkeys would not react with certain anti-human antibodies.  For example, I
had a couple of research animals that were negative for a CD3 antibody but
yet they stained with CD4 and CD8 antibodies.

Good luck!

Denise


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<https://rochediagnostic.sigstr.net/uc/56dd9d91825be9040100000b/watermark>*Denise
A Croix, Ph.D.*
Pathology Solutions Specialist - PD-L1 SP142 Assay
Roche Diagnostics Corporation
Tissue Diagnostics Division

972-207-7305
denise.croix at roche.com
www.ventana.com

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