[Histonet] ARC immunohistochemistry- "sticky sections" in DAB

[Gabrielle Pollack]

To whom it may concern,

I am a undergraduate researcher at Vassar College. I am performing
immunohistochemistry on mice using Arc primary, anti-mouse biotinylated IgG
(1:200),ABC (Vector labs) and DAB. So far we have gotten really great
staining, however the last immuno I ran, the sections, once in DAB were
sticking to the bottom of the wells. Does anyone have any idea as to why
this would happen? Thank you!

Best,
Gabrielle