[Histonet] CD34 for primate tissue

[Cathy M. Mathis/Comparative Medicine]

2 more bits of information about my dilemma;
I did try staining without any retrieval - no signal
I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer.
So I know the antibodies and my protocols work, just not on my rhesus.  Does anyone know of a CD34 that will work in this species?
More suggestions please?  Thank you all for your time.
Cathy

-----Original Message-----
From: Eddie Martin [mailto:edmartin26 at gmail.com] 
Sent: Wednesday, June 29, 2016 5:34 PM
To: Cathy M. Mathis/Comparative Medicine
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] CD34 for primate tissue

Hi Cathy,

EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. 

Best,
Eddie Martin, HTL, QIHC
Edmartin26 at gmail.com

Sent from my iPhone

> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine <cmmathis at wakehealth.edu> wrote:
> 
> Good morning my fellow Histo-netters,
> Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues?  A few companies have one with clone QEBend 10 and say that it works but I have had no luck.  I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica.  Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions.  Still no signal.  Any help would be greatly appreciated.
> Cathy
> 
> 
>