[Histonet] CD34 for primate tissue

[Eddie Martin]

Hi Cathy,

EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. 

Best,
Eddie Martin, HTL, QIHC
Edmartin26 at gmail.com

Sent from my iPhone

> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine <cmmathis at wakehealth.edu> wrote:
> 
> Good morning my fellow Histo-netters,
> Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues?  A few companies have one with clone QEBend 10 and say that it works but I have had no luck.  I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica.  Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions.  Still no signal.  Any help would be greatly appreciated.
> Cathy
> 
> 
>