[Histonet] CD34 for primate tissue

[Eddie Martin]

What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. 

Best,
Eddie Martin, HTL,QIHC

Sent from my iPhone

> On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine <cmmathis at wakehealth.edu> wrote:
> 
> 2 more bits of information about my dilemma;
> I did try staining without any retrieval - no signal
> I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer.
> So I know the antibodies and my protocols work, just not on my rhesus.  Does anyone know of a CD34 that will work in this species?
> More suggestions please?  Thank you all for your time.
> Cathy
> 
> -----Original Message-----
> From: Eddie Martin [mailto:edmartin26 at gmail.com] 
> Sent: Wednesday, June 29, 2016 5:34 PM
> To: Cathy M. Mathis/Comparative Medicine
> Cc: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] CD34 for primate tissue
> 
> Hi Cathy,
> 
> EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. 
> 
> Best,
> Eddie Martin, HTL, QIHC
> Edmartin26 at gmail.com
> 
> Sent from my iPhone
> 
>> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine <cmmathis at wakehealth.edu> wrote:
>> 
>> Good morning my fellow Histo-netters,
>> Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues?  A few companies have one with clone QEBend 10 and say that it works but I have had no luck.  I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica.  Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions.  Still no signal.  Any help would be greatly appreciated.
>> Cathy
>