[Histonet] CD34 for primate tissue

Cathy M. Mathis/Comparative Medicine cmmathis at wakehealth.edu
Wed Jun 29 08:16:16 CDT 2016


Good morning my fellow Histo-netters,
Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues?  A few companies have one with clone QEBend 10 and say that it works but I have had no luck.  I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica.  Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions.  Still no signal.  Any help would be greatly appreciated.
Cathy




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