[Histonet] CD34 for primate tissue
Cathy M. Mathis/Comparative Medicine
cmmathis at wakehealth.edu
Wed Jun 29 08:16:16 CDT 2016
Good morning my fellow Histo-netters,
Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated.
Cathy
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