[Histonet] block Scrapers
Hardy, Cate
chardy at csu.edu.au
Sun Jul 24 19:40:24 CDT 2016
We just use a blunt butcher knife. It works great. Low tech, low cost!
Cate Hardy
Senior Technical Officer (Mon-Tue) | Veterinary Enterprises, Faculty of Science
Charles Sturt University
Boorooma Street
Locked Bag 588
Wagga Wagga, NSW 2678
Australia
Tel: 69334000
Email: vdl at csu.edu.au
Email: chardy at csu.edu.au
www.csu.edu.au
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Sent: Saturday, 23 July 2016 3:00 AM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 152, Issue 20
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Today's Topics:
1. Unscribe (Fatima Formadi)
2. block scrapers (Lauren Sweeney)
3. Re: block scrapers (Jay Lundgren)
4. Re: block scrapers (Cooper, Brian)
5. Re: block scrapers (Elizabeth Chlipala)
6. Re: Reticulin Stain (taylor alan)
7. H&E staining on tape (Judi Ford)
8. Re: block scrapers (J B)
9. Re: block scrapers (Jennifer MacDonald)
10. Re: Reticulin Stain (Jennifer MacDonald)
11. Re: block scrapers (Rene J Buesa)
12. Crystals in PAS (Amos Brooks)
13. Re: block scrapers (Roberta Horner)
14. Air Bubbles (Charles Riley)
15. Re: block scrapers (Morken, Timothy)
----------------------------------------------------------------------
Message: 1
Date: Thu, 21 Jul 2016 12:17:50 -0600
From: Fatima Formadi <fatimaformadi at gmail.com>
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] Unscribe
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<CAJfbtJ=92KxBWNH8iWrQO1GEZ52Fa_cefe82KfY0p_YkgTLqJw at mail.gmail.com>
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Hi,
I wish to unscribe from this mailing list immediately please.
Thank you
Regards,
Fatima
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Message: 2
Date: Thu, 21 Jul 2016 18:46:53 +0000
From: Lauren Sweeney <lmarie08 at uga.edu>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] block scrapers
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Hi all,
My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me?
Thanks!!
L
------------------------------
Message: 3
Date: Thu, 21 Jul 2016 14:04:06 -0500
From: Jay Lundgren <jaylundgren at gmail.com>
To: Lauren Sweeney <lmarie08 at uga.edu>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<CANCZNuZBg4J4zffDkcivUtf4=oX+QRaa8MGA18qkvSjpqLYsmg at mail.gmail.com>
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Buy cheap paring knives at the dollar store and blunt the edges?
On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote:
> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 4
Date: Thu, 21 Jul 2016 19:08:02 +0000
From: "Cooper, Brian" <bcooper at chla.usc.edu>
To: "Jay Lundgren" <jaylundgren at gmail.com>, "Lauren Sweeney"
<lmarie08 at uga.edu>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<C12647AD4408834C8AB48FB0389C63E301A6CBBDFA at CHLAEXMBH01.LA.AD.CHLA.ORG>
Content-Type: text/plain; charset=us-ascii
I bought everyone really old pocket knives from flea markets--basically the same thing as what Jay said. For the most part, these old knives were already really dull and didn't require any assistance to dull them further.
Good luck.
Brian
-----Original Message-----
From: Jay Lundgren via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 12:04 PM
To: Lauren Sweeney
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] block scrapers
Buy cheap paring knives at the dollar store and blunt the edges?
On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote:
> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Message: 5
Date: Thu, 21 Jul 2016 13:09:13 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: Lauren Sweeney <lmarie08 at uga.edu>,
"'histonet at lists.utsouthwestern.edu'
(histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
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Lauren
I have always used a very dull steak knife (sharp ones are too sharp and will cut the cassette) mine is probably over 20 years old (now I'm dating myself). I'm old school so I still use that but the rest of the techs will use the para trimmer. The para trimmer is more ergonomically friendly less repetitive than a knife - here is the link to the website
https://www.thermofisher.com/order/catalog/product/B3120205
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 12:47 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] block scrapers
Hi all,
My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me?
Thanks!!
L
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Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 21 Jul 2016 20:30:31 +0100 (BST)
From: taylor alan <aj.taylor at blueyonder.co.uk>
To: Rene J Buesa <rjbuesa at yahoo.com>, Anita Buchiane
<abuchiane at bmhvt.org>, Rene J Buesa via Histonet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Reticulin Stain
Message-ID:
<846648922.1768040.1469129431802.JavaMail.open-xchange at oxbe1.tb.ukmail.iss.as9143.net>
Content-Type: text/plain; charset=UTF-8
Hi All
Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red.
We still prefer to prepare our own in-house solutions and reagents rather than purchase expensive commercial products. Old fashioned I know! But this is the common way still in many small, independent UK labs.
Histonet is such a valuable resource. I look forward to reading daily submissions and comments.
Alan Taylor
Microtechnical Services
Exeter. Devon. UK
>
> On 21 July 2016 at 17:09 Rene J Buesa via Histonet
> <histonet at lists.utsouthwestern.edu> wrote:
>
>
> Gomori's Ren?
>
> On Thursday, July 21, 2016 12:04 PM, Anita Buchiane via Histonet
> <histonet at lists.utsouthwestern.edu> wrote:
>
>
> Does anyone out there still do the retic by hand? If so, can you
> share which procedure you use? Thanks
>
>
>
>
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------------------------------
Message: 7
Date: Thu, 21 Jul 2016 19:31:17 +0000
From: Judi Ford <jford at cytomx.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] H&E staining on tape
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Hi everyone,
I have a colleague that has been asked to do H&E staining on a frozen specimen collected on tape. The tape has been attached to the slide using nail polish. Can't use the CryoJane system due to the light flash. Her question is "will the nail polish disintegrate during the immersion in alcohol and xylene?". Another question is will the stains attach to the tape and obscure the tissue? If so, what to do with about that. She is also worried that the tape will fall off into the staining dish and get lost. I'm thinking maybe using a 6 well disposable tissue culture plate for the staining of the tape then reattach it to the slide and coverslip.
Any suggestions?
Thanks so much,
Judi
------------------------------
Message: 8
Date: Thu, 21 Jul 2016 19:32:48 +0000
From: J B <craigak12 at gmail.com>
To: Lauren Sweeney <lmarie08 at uga.edu>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<CANyXu7DCtN2_68ScUsrXP7bXQqHxz8SRrmjefYBJ6tYjcZQ-aw at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Yes, search paratrimmer you will love it. It melts excess paraffin off blocks.
JB
On Thu, Jul 21, 2016, 11:52 AM Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote:
> Hi all,
>
> My lab is in need of some tools to scrap the paraffin off the edges of
> the blocks after embedding. Does anyone have any recommendations for me?
>
>
> Thanks!!
> L
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Thu, 21 Jul 2016 12:45:55 -0700
From: Jennifer MacDonald <JMacDonald at mtsac.edu>
To: J B <craigak12 at gmail.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>,Lauren Sweeney <lmarie08 at uga.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<OF74D0809B.B833609D-ON88257FF7.006C930B-88257FF7.006C930D at mtsac.edu>
Content-Type: text/plain; charset="UTF-8"
------------------------------
Message: 10
Date: Thu, 21 Jul 2016 12:51:41 -0700
From: Jennifer MacDonald <JMacDonald at mtsac.edu>
To: Anita Buchiane <abuchiane at bmhvt.org>
Cc: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Reticulin Stain
Message-ID:
<OF68E3CB71.85106924-ON88257FF7.006D1A15-88257FF7.006D1A16 at mtsac.edu>
Content-Type: text/plain; charset="UTF-8"
------------------------------
Message: 11
Date: Thu, 21 Jul 2016 20:52:26 +0000 (UTC)
From: Rene J Buesa <rjbuesa at yahoo.com>
To: Lauren Sweeney <lmarie08 at uga.edu>,
"histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<1022675503.2796010.1469134346522.JavaMail.yahoo at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Use a regular one blade pocket knife (as?I used to do).Ren?
On Thursday, July 21, 2016 2:49 PM, Lauren Sweeney via Histonet <histonet at lists.utsouthwestern.edu> wrote:
Hi all,
My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me?
Thanks!!
L
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Fri, 22 Jul 2016 04:52:37 -0400
From: Amos Brooks <amosbrooks at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Crystals in PAS
Message-ID:
<CAC95ki-qdcQ-3g+5b+1SCoorn+K5WteuKn4LP7PLKO9ctWze3A at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hi,
If you are having trouble with the instrument and the manufacturer isn't able to fix it, just hand stain them. PAS is really not a complicated stain. Please don't just accept underperforming equipment when we are all capable of so much more.
Amos
------------------------------
Message: 13
Date: Fri, 22 Jul 2016 14:14:48 +0000
From: Roberta Horner <rjr6 at psu.edu>
To: "Histonet (histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID: <922f26cac06547eeb147719d6354acd5 at PSU.EDU>
Content-Type: text/plain; charset="iso-8859-1"
I use a single edge razor blade that I've opened boxes with and is dulled to just the right sharpness. And I wish people would quit throwing it away Roberta Horner Animal Diagnostic Lab Penn State
------------------------------
Message: 14
Date: Fri, 22 Jul 2016 10:42:50 -0400
From: Charles Riley <criley at dpspa.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Air Bubbles
Message-ID:
<CAAQhB10DuzuOEenADLe=TWoudm71kkT8SEkm68Ki9rqO+cxX7Q at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Has anyone encountered the following problem.
Tissue cut and is processed well. There is no distortion at a cellular level. However anywhere from 30 to 2 days later there are air bubbles under the coverslip. I know for certain there aren't even the slightest bit of air bubbles when coverslipped. I have a feeling it could still be a processing issue as I ran a few tests like coverslipping blank slides and tissue sections from a year ago when there was no issues. If anyone has any suggestions as to what the problem could be and potential solutions please get back to me as soon as possible as this is becoming a major issue for my lab.
--
Charles Riley HT(ASCP)CM
Histopathology Coordinator/ Mohs
------------------------------
Message: 15
Date: Fri, 22 Jul 2016 15:29:58 +0000
From: "Morken, Timothy" <Timothy.Morken at ucsf.edu>
To: Lauren Sweeney <lmarie08 at uga.edu>
Cc: Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] block scrapers
Message-ID:
<761E2B5697F795489C8710BCC72141FF6FD6B2BE at ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Sakura has purpose-made scrapes. Otherwise you can use plastic scrapers from a hardware store.
However, we moved away from scrapers and now use a heating block trimmer made for the purpose. Several vendors offer these.
http://www.newcomersupply.com/product/paraffin-wax-trimmer
We do all block trimming at a central location so it does not need to be done at the microtome.
It is very quick and does not damage the print on the block. That is especially important for barcoded blocks.
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center
-----Original Message-----
From: Lauren Sweeney via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2016 11:47 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] block scrapers
Hi all,
My lab is in need of some tools to scrap the paraffin off the edges of the blocks after embedding. Does anyone have any recommendations for me?
Thanks!!
L
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