[Histonet] H&E staining

[Teri Johnson]

Hi Charles,

If my memory serves, Clarifier 1 and Clarifier 2 are different mostly in that one is an alcoholic solution and the other an aqueous one. So they both behave differently in their mechanism of action. The alcohol based reagent should remove more hematoxylin from the cells than the aqueous based reagent.

Also if you do a google search for Richard Allan Clarifier 1, you should find a pdf document that outlines what their stain series are, and how to use (time) them. It's a great document that has a couple charts on how to increase or decrease stain contrast.  It appears that they recommend 30 seconds-1 minute for either solution for the Hematoxylin 7211 and 7212 stain, and 20 seconds-1 minute if using the Hematoxylin 1 and 2. At 2.5 minutes, you might be overdoing your "clarifying"!

Best wishes and good luck!

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com<mailto:tejohnson at genoptix.com>
www.genoptix.com<http://www.genoptix.com/>


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