[Histonet] H & E Troubleshooting

[Michael Ann Jones]

Watch your soap residue in the staining dishes. That will mess you up.
Bleach does leave a residue, that¹s why we then wash in soap.
But if you wash in soap you must use a soap residue measurement to prove
the soap is rinsed clean.
It will change the pH of your Heme. Spent a long 6 months figuring that
out!!
Such a subtle influence.
Good luck!

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones at metropath.com






On 2/23/16, 11:11 AM, "Cassie P. Davis via Histonet"
<histonet at lists.utsouthwestern.edu> wrote:

>Hi Histo folks,
>
>         We are having a fun time troubleshooting our H&E because the
>problem in not consistent...
>
>
>
>Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our
>alcohol since our ordered had not arrived yet. One of our doctor said our
>hematoxylin & eosin was readable but too light(he got 6 out of the 36
>cases), the other (who got the 30 cases)said it was good.
>
>
>
>We had half the number of slides going through staining than we normally
>do.
>
>We don't let the last alcohol before xylene get pink from eosin.
>
>Had it been a Thursday-Friday I would be concerned the xylene needed to
>be changed.
>
>The Define and Bluing were made fresh.
>
>
>
>My theory is the tap water could be off or the xylene needs to be changed.
>
>Another theory floating in the lab is because we bleach the stain buckets
>over the weekend maybe the bleach is saturating the bucket and slowly
>leaching out diluting the hematoxylin later in the day. (I would think
>this would be the case for all the slides not just the last few)
>
>
>Cassandra Davis
>Histology Technician
>Anatomical Pathology Laboratory
>Saint Francis Healthcare
>701 N. Clayton Street
>Wilmington,DE 19805
>Office:  302-575-8095
>Email:  CDavis at che-east.org<mailto:n at che-east.org>
>www.saintfrancishealthcare.org
>
>
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