[Histonet] Glassware Cleaning

[P Sicurello]

We use the bromocresol and it does stay yellow if the pH is correct.  We
also use a narrow range pH strip.  You might want to check the pH of your
DI water.




Sincerely,



Paula



Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Mon, Feb 1, 2016 at 1:54 PM, Samantha Sackmann via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> I have tried this procedure multiple times and everytime the solution
> turns purple in the glassware filled with deionized water, even after
> rinsing in running water for 2+ minutes.  We do not have a dishwasher, so
> all the glassware is hand-washed.  Could there be a problem with our DI
> water? Has anyone else had an issue with this?
>
> Thanks,
> Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II
> CellNetix Pathology & Laboratories
> 800 West 5th Avenue
> Spokane, WA 99204
> 509-473-2311
> sedwards at cellnetix.com
>
>
>
>
>
>
>
>
>
> What is everyone using to test their glassware for detergent residue? Is
> there a ready to use solution or not?
>
>
>
>
>
> CAP Checklist...
>
>
>
> Glassware Cleaning Phase II
>
>
>
> There are appropriate documented procedures for handling and cleaning
> glassware, including
>
> methods for testing for detergent removal.
>
>
>
> NOTE: Special instructions for micropipettes, cuvets, acid washing, etc.
> must be included.
>
>
>
> A simple procedure to check for detergent residue uses bromcresol purple
> (0.1 g bromcresol purple
>
> in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled
> water into a representative,
>
> washed, glassware item. Add two to three drops bromcresol solution. A
> purple color reveals residual
>
> detergent. A yellow color indicates satisfactory rinsing.
>
>
>
> Thanks!
>
>
>
>
>
> Matthew Roark- HT/HTL(ASCP)CM
>
> Histology Specialist
>
> Saint Francis Medical Center
>
> 211 Saint Francis Drive
>
> Cape Girardeau, MO 63703
>
> 573-331-3982
>
> mroark <@t> sfmc.net<
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
>
> http://www.sfmc.net<http://www.sfmc.net/>
>
>
>
>
> Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II
> CellNetix Pathology & Laboratories
> 800 West 5th Avenue
> Spokane, WA 99204
> 509-473-2311
> sedwards at cellnetix.com
>
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>
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