[Histonet] Histonet Digest, Vol 147, Issue 1

Tue Feb 2 09:01:28 CST 2016

Hi
I have a question, I need to find out the pay scale for a certified & non certified histologist in Southern California. Thanks

Aloha Lisa
Sent from my iPhone

> On Feb 1, 2016, at 10:00 AM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
> 
>   1. Storage of Unstained Slides (T Williams)
>   2. Destaining IHC's (Estela Martinez)
>   3. FW:  Storage of Unstained Slides (Hannen, Valerie)
>   4. Re: Storage of Unstained Slides (WILLIAM DESALVO)
>   5. Salutations from former GC histo sci student (Roy, Ryan)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Mon, 1 Feb 2016 07:23:06 -0500
> From: T Williams <wtaylor8660 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Storage of Unstained Slides
> Message-ID:
>    <CANmnUpYaAztLaFGZWwuPekxoSNaB3NUp4P8BNES=i1gTt7YVFQ at mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
> 
> Does anyone have any suggestions as to how unstained slides with patient
> tissue should be stored?  Or could anyone recommend a product for
> storage?   Also - is it preferable that these slides not be placed in the
> dryer until they are intended to be stained?  If so, what is the problem
> with baking them as soon as they're cut and dried at room temperature?
> 
> I apologize for all the questions but I do  appreciate the guidance.
> 
> T. Williams
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Mon, 1 Feb 2016 15:18:59 +0000
> From: Estela Martinez <emartinez2 at echd.org>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Destaining IHC's
> Message-ID:
>    <BY1PR0501MB1205CA5796EF85E0225EC2BAE1DE0 at BY1PR0501MB1205.namprd05.prod.outlook.com>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Good Morning ALL,
> 
> Do anybody have a protocol on destaing IHC's?  Is it possible to do that?  Need some help!
> 
> 
> Estela Martinez
> Histology Supervisor
> Medical Center Hospital
> Odessa, TX 79761
> 432-640-2348
> emartinez2 at echd.org
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> 
> ------------------------------
> 
> Message: 3
> Date: Mon, 1 Feb 2016 10:43:29 -0500
> From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
> To: "Histonet at lists.utsouthwestern.edu"
>    <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] FW:  Storage of Unstained Slides
> Message-ID: <450B7A81EDA0C54E97C53D60F00776C323546750B2 at isexstore03>
> Content-Type: text/plain; charset="us-ascii"
> 
> 
> 
> -----Original Message-----
> From: Hannen, Valerie 
> Sent: Monday, February 01, 2016 10:41 AM
> To: 'T Williams'
> Subject: RE: [Histonet] Storage of Unstained Slides
> 
> We store our unstained slides in the drawer with the H&E Slides. We do bake them at @ 64 degrees Celsius for @ 20 minutes  before storing them .
> 
> 
> 
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.hannen at parrishmed.com
> www.parrishmed.com
> 
> 
> 
> -----Original Message-----
> From: T Williams via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
> Sent: Monday, February 01, 2016 7:23 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Storage of Unstained Slides
> 
> Does anyone have any suggestions as to how unstained slides with patient tissue should be stored?  Or could anyone recommend a product for
> storage?   Also - is it preferable that these slides not be placed in the
> dryer until they are intended to be stained?  If so, what is the problem with baking them as soon as they're cut and dried at room temperature?
> 
> I apologize for all the questions but I do  appreciate the guidance.
> 
> T. Williams
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> 
> ------------------------------
> 
> Message: 4
> Date: Mon, 1 Feb 2016 08:55:47 -0700
> From: WILLIAM DESALVO <wdesalvo.cac at outlook.com>
> To: T Williams <wtaylor8660 at gmail.com>,
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Storage of Unstained Slides
> Message-ID: <COL402-EAS31268E064D06CC2BB40126B82DE0 at phx.gbl>
> Content-Type: text/plain; charset="utf-8"
> 
> I would not heat dry unstained as heat drying removes the paraffin and cells/tissue in no longer protected by the paraffin. Drying slides is a process to remove the sate trapped between the section and glass. Heat drying can speed that process and is also helpful in "baking" tissue to slide to assist in tissue adherence. Air dry until staining is requested, is my advice.
> 
> Sent from my Windows Phone
> ________________________________
> From: T Williams via Histonet<mailto:histonet at lists.utsouthwestern.edu>
> Sent: ?2/?1/?2016 5:31 AM
> To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Storage of Unstained Slides
> 
> Does anyone have any suggestions as to how unstained slides with patient
> tissue should be stored?  Or could anyone recommend a product for
> storage?   Also - is it preferable that these slides not be placed in the
> dryer until they are intended to be stained?  If so, what is the problem
> with baking them as soon as they're cut and dried at room temperature?
> 
> I apologize for all the questions but I do  appreciate the guidance.
> 
> T. Williams
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Mon, 1 Feb 2016 11:42:06 -0500
> From: "Roy, Ryan" <Ryan.Roy at va.gov>
> To: 'Kelli Goodkowsky via Histonet'
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Salutations from former GC histo sci student
> Message-ID:
>    <15F883394EAB744E99E1C7E1B9873049018C3256BFE2 at R04BYNMSGB1.r04.med.va.gov>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello Kelli,
> 
> You may remember me as one of your online graduates from the GC histological science program. How are you? For some reason I thought your involvement with the GC histo program had changed, but seeing your recent post to the histonet it appears your still the director of that program?
> 
> I hope things are well. You were instrumental in helping me complete that program successfully and were also a good instructor. Ive been at the VA Manchester NH for 2 years now. I am the lone histotech here since the other technician left. Things are going great though we have a critical need for  another qualified technician.  Please let me know if any of your students especially those already holding  a BS would be interested in employment at the VA Hospital  in Manchester NH. I could certainly guide them through the application process.
> 
> Thanks a ton,
> 
> 
> Ryan Roy HTL (ASCP)
> Histology Lab
> Manchester VA
> 718 Symth Rd
> Manchester NH 03104
> (603) 624-4366 ex 6640
> 
> 
> Disclosure: The content of this email does not reflect the policies, views or opinions of the VA.
> 
> 
> 
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> ------------------------------
> 
> End of Histonet Digest, Vol 147, Issue 1
> ****************************************