[Histonet] Glassware Cleaning

[Samantha Sackmann]

I have tried this procedure multiple times and everytime the solution turns purple in the glassware filled with deionized water, even after rinsing in running water for 2+ minutes.  We do not have a dishwasher, so all the glassware is hand-washed.  Could there be a problem with our DI water? Has anyone else had an issue with this?

Thanks,
Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II
CellNetix Pathology & Laboratories
800 West 5th Avenue
Spokane, WA 99204
509-473-2311
sedwards at cellnetix.com









What is everyone using to test their glassware for detergent residue? Is there a ready to use solution or not?





CAP Checklist...



Glassware Cleaning Phase II



There are appropriate documented procedures for handling and cleaning glassware, including

methods for testing for detergent removal.



NOTE: Special instructions for micropipettes, cuvets, acid washing, etc. must be included.



A simple procedure to check for detergent residue uses bromcresol purple (0.1 g bromcresol purple

in 50 mL ethyl alcohol). Pipette approximately 5 cm (2 inches) distilled water into a representative,

washed, glassware item. Add two to three drops bromcresol solution. A purple color reveals residual

detergent. A yellow color indicates satisfactory rinsing.



Thanks!





Matthew Roark- HT/HTL(ASCP)CM

Histology Specialist

Saint Francis Medical Center

211 Saint Francis Drive

Cape Girardeau, MO 63703

573-331-3982

mroark <@t> sfmc.net<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>

http://www.sfmc.net<http://www.sfmc.net/>




Samantha Sackmann, CG(ASCP)CMHTLCM, Histotechnologist II
CellNetix Pathology & Laboratories
800 West 5th Avenue
Spokane, WA 99204
509-473-2311
sedwards at cellnetix.com

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