[Histonet] Eosin

[E. Wayne Johnson 朱稳森博士]

You might try some combination of eosin and biebrich scarlet and/or 
phloxine.

Adding a little acetic acid might help.

http://www.stainsfile.info/StainsFile/downloads/eosin.pdf

I am quite fond of Biebrich Scarlet and like way it stains brain and 
heart particularly.

Biebrich is much more red than eosin.

Phloxine is an aggressive pink.

*

A bit punny about the fish looking washed out.

*

"There was something fishy about the butler.

Probably a Pisces working for scale." - Phil Proctor



On 08/30/2016 10:25 PM, Cameron, Elizabeth wrote:
> Hi,
> I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense.  Our standard protocol works well for our own tissue, but the fish look much more washed out.  I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin.  I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic.  Any suggestions would be appreciated.
> Thank you.
>
> Elizabeth M. Cameron, HT(ASCP), QIHCCM
> Lead Histologist
> Mid Coast Hospital
> 123 Medical Center Drive
> Brunswick, ME 04011
> (207) 373-6573
>
>

-- 
E. Wayne Johnson 朱稳森博士
Enable AgTech Consulting
恩睿康农业技术咨询有限公司
Beijing
188 1088 3205