[Histonet] Eosin

[Debbie Faichney]

Hi Elizabeth,

We are an aquaculture histology laboratory and routinely use four parts 1% Eosin Y (aq) to one part Putts eosin for the same reason that you describe.  1% aqueous or alcoholic alone just doesn't do the job.

Debbie Faichney, BSc

Senior Technician
Histology/Bacteriology Laboratories
Institute of Aquaculture
University of Stirling
Stirling, FK9 4LA
Scotland
UK








-----Original Message-----
From: Cameron, Elizabeth via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: 30 August 2016 15:25
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Eosin

Hi,
I have been staining fish tissues fixed in Davidsons with H&E, and the researcher would like the eosin to be more intense.  Our standard protocol works well for our own tissue, but the fish look much more washed out.  I am using alcoholic eosin Y, have tried both water and alcohol before and I have varied the alcohol differentiation steps after the Eosin.  I also extended the time in Eosin and increased the wash after bluing to make sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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