[Histonet] Eosin

Jennifer MacDonald JMacDonald at mtsac.edu
Tue Aug 30 13:09:00 CDT 2016

You can try to lower the pH of the eosin a bit.  Add some acetic acid. Use 
absolute alcohol after the eosin for differentiating/dehydrating.

From:   "Cameron, Elizabeth via Histonet" 
<histonet at lists.utsouthwestern.edu>
To:     "histonet at lists.utsouthwestern.edu" 
<histonet at lists.utsouthwestern.edu>
Date:   08/30/2016 07:29 AM
Subject:        [Histonet] Eosin

I have been staining fish tissues fixed in Davidsons with H&E, and the 
researcher would like the eosin to be more intense.  Our standard protocol 
works well for our own tissue, but the fish look much more washed out.  I 
am using alcoholic eosin Y, have tried both water and alcohol before and I 
have varied the alcohol differentiation steps after the Eosin.  I also 
extended the time in Eosin and increased the wash after bluing to make 
sure the sections are not basic.  Any suggestions would be appreciated.
Thank you.

Elizabeth M. Cameron, HT(ASCP), QIHCCM
Lead Histologist
Mid Coast Hospital
123 Medical Center Drive
Brunswick, ME 04011
(207) 373-6573

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