[Histonet] Hematoxylin Precipitate

[nancy lowen]

Yes, I have had the same experience.Filtering  it seems to help. 


     On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet <histonet at lists.utsouthwestern.edu> wrote:
   

 Hello all,

                Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.

                Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome.

Cheers,

Sandy

 

Sandra J. Cheasty, HT (ASCP)

Histology & Necropsy Supervisor

UW-Madison, School of Veterinary Medicine

 

 

 

 



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