[Histonet] Hematoxylin Precipitate

[Michael Ann Jones]

Yes, me too.
Turquois blue bacteria looking guys.
Trying to filter and use within a couple of days.
Has anyone mentioned this to their vendor?

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones at metropath.com







On 9/21/15, 3:17 PM, "nancy lowen via Histonet"
<histonet at lists.utsouthwestern.edu> wrote:

>Yes, I have had the same experience.Filtering  it seems to help.
>
>
>     On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet
><histonet at lists.utsouthwestern.edu> wrote:
>   
>
> Hello all,
>
>                Has anyone using Richard Allen Hematoxylin-2 noticed an
>odd artifact on the slides after using the Hematoxylin for more than a
>few days on their stainer? We are seeing small spore or pollen-like blue
>dots here and there on the slides. It is not coming from the water bath
>or our water supply on the stainer. I used sterile gloves, opened a new
>case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on
>the stainer, then in DI again, air-dried and coverslipped them, and the
>blue dots were there. The only way we got rid of the blue artifact was to
>use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.
>
>                Thanks for your input, and if you can recommend a
>different, reasonably priced hematoxylin, that would be awesome.
>
>Cheers,
>
>Sandy
>
> 
>
>Sandra J. Cheasty, HT (ASCP)
>
>Histology & Necropsy Supervisor
>
>UW-Madison, School of Veterinary Medicine
>
> 
>
> 
>
> 
>
> 
>
>
>
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