[Histonet] Hematoxylin Precipitate

[Sandra Cheasty]

Hello all,

                Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive.

                Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome.

Cheers,

Sandy

 

Sandra J. Cheasty, HT (ASCP)

Histology & Necropsy Supervisor

UW-Madison, School of Veterinary Medicine