[Histonet] Recommendations of places to purchase two stains and question on protocol

[Elizabeth Chlipala]

Jorge

I can comment on the cresyl etch violet - we actually purchased  cresyl violet acetate from MP Biomedicals.  This particular chemical has not been certified by the biological stain commission, if you are looking for a dye that has been certified then I would go to Sigma-Aldrich - they also supply this chemical.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Jorge A. Santiago-Blay via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Friday, November 27, 2015 9:58 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Recommendations of places to purchase two stains and question on protocol

Dear Histonetters:


I am trying to purchase two stains:

1. Cresil etch violet (for nervous tissue) - https://en.wikipedia.org/wiki/Cresyl_violet
<https://webaccess.si.edu/owa/redir.aspx?SURL=MM5iL3kv__yYURBC3BJzcrSdOSOKhjUXtHfvftfASlIloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwBlAG4ALgB3AGkAawBpAHAAZQBkAGkAYQAuAG8AcgBnAC8AdwBpAGsAaQAvAEMAcgBlAHMAeQBsAF8AdgBpAG8AbABlAHQA&URL=https%3a%2f%2fen.wikipedia.org%2fwiki%2fCresyl_violet>



2. Mallory azan (for encocrine tissue)  - https://www.emsdiasum.com/microscopy/technical/datasheet/26450.aspx
<https://webaccess.si.edu/owa/redir.aspx?SURL=Shojbo1T3JKV6jfR3tNAKE-6WcR1vrPob5zt6rdIorEloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwB3AHcAdwAuAGUAbQBzAGQAaQBhAHMAdQBtAC4AYwBvAG0ALwBtAGkAYwByAG8AcwBjAG8AcAB5AC8AdABlAGMAaABuAGkAYwBhAGwALwBkAGEAdABhAHMAaABlAGUAdAAvADIANgA0ADUAMAAuAGEAcwBwAHgA&URL=https%3a%2f%2fwww.emsdiasum.com%2fmicroscopy%2ftechnical%2fdatasheet%2f26450.aspx>
 ,

 https://en.wikipedia.org/wiki/Heidenhain%27s_AZAN_trichrome_stain
<https://webaccess.si.edu/owa/redir.aspx?SURL=IbvXghzm4CDaxFzSUM5VXbFYnPu9CO_b0NANiXpbVVgloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwBlAG4ALgB3AGkAawBpAHAAZQBkAGkAYQAuAG8AcgBnAC8AdwBpAGsAaQAvAEgAZQBpAGQAZQBuAGgAYQBpAG4AJQAyADcAcwBfAEEAWgBBAE4AXwB0AHIAaQBjAGgAcgBvAG0AZQBfAHMAdABhAGkAbgA.&URL=https%3a%2f%2fen.wikipedia.org%2fwiki%2fHeidenhain%2527s_AZAN_trichrome_stain>

Have searched the web and rather than gamble, wish to know if I could receive recommendations on good places to purchase this type of reagent.
Good customer service and affordable prices a must.

In both cases, do you know if these stains work on tissues preserved in ethanol (70%), some of the tissues could have been preserved for years.

If you have constructive feedback, please feel free to email me at

blayjorge at gmail.com

Gratefully,

Jorge


Jorge A. Santiago-Blay, PhD
blaypublishers.com

1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/

2. Free examples of papers published in *LEB*:
http://blaypublishers.com/category/previous-issues/.

3. *Guidelines for Authors* and page charges of *LEB*:
http://blaypublishers.com/archives/ *.*

4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/


http://blayjorge.wordpress.com/
http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet