[Histonet] Recommendations of places to purchase two stains and question on protocol

Jorge A. Santiago-Blay blayjorge at gmail.com
Fri Nov 27 10:58:07 CST 2015


Dear Histonetters:


I am trying to purchase two stains:

1. Cresil etch violet (for nervous tissue) -
https://en.wikipedia.org/wiki/Cresyl_violet
<https://webaccess.si.edu/owa/redir.aspx?SURL=MM5iL3kv__yYURBC3BJzcrSdOSOKhjUXtHfvftfASlIloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwBlAG4ALgB3AGkAawBpAHAAZQBkAGkAYQAuAG8AcgBnAC8AdwBpAGsAaQAvAEMAcgBlAHMAeQBsAF8AdgBpAG8AbABlAHQA&URL=https%3a%2f%2fen.wikipedia.org%2fwiki%2fCresyl_violet>



2. Mallory azan (for encocrine tissue)  -
https://www.emsdiasum.com/microscopy/technical/datasheet/26450.aspx
<https://webaccess.si.edu/owa/redir.aspx?SURL=Shojbo1T3JKV6jfR3tNAKE-6WcR1vrPob5zt6rdIorEloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwB3AHcAdwAuAGUAbQBzAGQAaQBhAHMAdQBtAC4AYwBvAG0ALwBtAGkAYwByAG8AcwBjAG8AcAB5AC8AdABlAGMAaABuAGkAYwBhAGwALwBkAGEAdABhAHMAaABlAGUAdAAvADIANgA0ADUAMAAuAGEAcwBwAHgA&URL=https%3a%2f%2fwww.emsdiasum.com%2fmicroscopy%2ftechnical%2fdatasheet%2f26450.aspx>
 ,

 https://en.wikipedia.org/wiki/Heidenhain%27s_AZAN_trichrome_stain
<https://webaccess.si.edu/owa/redir.aspx?SURL=IbvXghzm4CDaxFzSUM5VXbFYnPu9CO_b0NANiXpbVVgloNx7SvfSCGgAdAB0AHAAcwA6AC8ALwBlAG4ALgB3AGkAawBpAHAAZQBkAGkAYQAuAG8AcgBnAC8AdwBpAGsAaQAvAEgAZQBpAGQAZQBuAGgAYQBpAG4AJQAyADcAcwBfAEEAWgBBAE4AXwB0AHIAaQBjAGgAcgBvAG0AZQBfAHMAdABhAGkAbgA.&URL=https%3a%2f%2fen.wikipedia.org%2fwiki%2fHeidenhain%2527s_AZAN_trichrome_stain>

Have searched the web and rather than gamble, wish to know if I could
receive recommendations on good places to purchase this type of reagent.
Good customer service and affordable prices a must.

In both cases, do you know if these stains work on tissues preserved in
ethanol (70%), some of the tissues could have been preserved for years.

If you have constructive feedback, please feel free to email me at

blayjorge at gmail.com

Gratefully,

Jorge


Jorge A. Santiago-Blay, PhD
blaypublishers.com

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