[Histonet] DIF on paraffin embedded tissue

[Anatoli Gleiberman]

Rene,
For many (but not for all) antigens with proper fixation (not too long) and retrieval IF on FFPE sections works fine.
 Here is short list of what we are doing routinely: all pituitary hormones, smooth muscle actin, alpha-fetoprotein, BJP, Ki67, p65 NFkB, CD3, IgG subclasses, IgM, B220, SOD2, POU domain transcription factors. Of course, fresh frozen samples or frozen sections from formaldehyde fixed samples give much better results, but still - if you need to do it on FFPE it is possible.

Anatoli Gleiberman, PhD
Director of Histopathology
Buffalo Biolabs LLC,
73 High Street
Buffalo, NY, 14203

Phone:716-849-6810x354
e-mail:agleiberman at buffalobiolab.com


-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 11:02 AM
To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René 


    On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 We have a tissue sample that was processed and paraffin embedded.  We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample.
 Does anyone have a procedure.  HELP
  
    madeathridge at pastnashville.com  

  




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