[Histonet] DIF on paraffin embedded tissue

[Simmons, Christopher]

By all means share your protocols plz

Sent from my iPhone

> On Nov 25, 2015, at 12:44 PM, Houston, Ronald via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Obviously it depends on what you are looking at, but I have had much success with DIF on paraffin sections
> 
> 
> Ronnie Houston, MS HT(ASCP)QIHC FIBMS
> Anatomic Pathology Manager
> 700 Children's Drive
> Columbus, OH 43205
> (P) 614-722-5450
> (F) 614-722-2899
> ronald.houston at nationwidechildrens.org
> www.NationwideChildrens.org
> 
> "Without continual growth and progress, such words as improvement, achievement, and success have no meaning."
> ~ Ben Franklin
> 
> 
> 
> -----Original Message-----
> From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
> Sent: Wednesday, November 25, 2015 11:02 AM
> To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] DIF on paraffin embedded tissue
> 
> No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René 
> 
> 
>    On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> 
> We have a tissue sample that was processed and paraffin embedded.  We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample.
> Does anyone have a procedure.  HELP
>   
>     madeathridge at pastnashville.com  
> 
>   
> 
> 
> 
> 
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