[Histonet] DIF on paraffin embedded tissue

Houston, Ronald Ronald.Houston at nationwidechildrens.org
Wed Nov 25 11:43:41 CST 2015


Obviously it depends on what you are looking at, but I have had much success with DIF on paraffin sections


Ronnie Houston, MS HT(ASCP)QIHC FIBMS
Anatomic Pathology Manager
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston at nationwidechildrens.org
www.NationwideChildrens.org

"Without continual growth and progress, such words as improvement, achievement, and success have no meaning."
~ Ben Franklin



-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 11:02 AM
To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] DIF on paraffin embedded tissue

No matter WHO to tell you to do WHAT, for IF purposes, that FFPE tissue is USELESS.René 


    On Wednesday, November 25, 2015 10:55 AM, Maryann Deathridge via Histonet <histonet at lists.utsouthwestern.edu> wrote:
 

 We have a tissue sample that was processed and paraffin embedded.  We URGENTLY need to recover the tissue and perform Immunofluorescence on the sample.
 Does anyone have a procedure.  HELP
  
    madeathridge at pastnashville.com  

  




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