[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Colleen Forster]

I am interested to see what you hear Brett....I need to do these same
markers and am not having good luck in FFPE....frozens , great...FFPE NOT!

C

On Tue, Nov 3, 2015 at 7:32 AM, Connolly, Brett M via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi all,
> I have a project that will be looking at CD8 expression in some FFPE
> murine tissue.  Past discussions here have mentioned the 14-0808 CD8a
> antibody from eBiosicence.
>
> For those of you have had the opportunity to use this antibody I would
> like to hear your experiences/ recommendations about the staining results.
>
> I'll need to do some quantitation of the staining so I am hoping this
> antibody is strong and specific.
>
> Thanks much,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly at merck.com<mailto:brett_connolly at merck.com>
> T- 215-652-2501
> F- 215-993-6803
>
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