[Histonet] CD8 IHC on FFPE mouse tissue -what your experience?

[Erickson, Jamie E]

Hi Brett,
We love this CD8 and the CD4  on FFPE samples we have  tested them  on mouse spleen, thymus, and small intestine. The CD8a stains nicely at 0.075ug/ml.
That being said we use a Leica BOND RX to stain our samples which has a Rabbit IgG polymer detection which amplifies our signal.
 We do a heat step called H2 20 which is ( EDTA) for 20 minutes at ~ 95C.
Primary antibody concentration 0.075ug/ml 30 min incubation
Secondary antibody Rabbit anti-Rat Vector labs cat # AI-4001 concentration 10ug/ml incubation 20 minutes.
I uploaded an image at: http://histosearch.com/imageupload/

We have not done any quantitation with this yet but I'm sure it would work nicely.

Jamie Erickson
Scientist
Foundational Immunology
Abbvie Laboratories
Worcester, MA 01605
Jamie.erickson at abbvie.com<mailto:Jamie.erickson at abbvie.com>
T-508-688-3134