[Histonet] PAS-diastase staining and formalin "crystals"

Julio Benavides j.benavides at eae.csic.es
Fri May 29 10:30:57 CDT 2015


Hi there,

thank you all very much for your comments and protocols on the 
PAS-diastase. Although funny thing that cheaper option, I think I stick 
to the Sigma enzyme first.

Update on the formalin "crystals". It is actually, as you suggested, 
white granular precipitate. There was so much of it that it formed some 
big "crusts" (which led me to think about crystals when looking trough 
the bottle...). The formalin was aqueous solution (stabilised  with 10% 
methanol). I have tried to depolymerize it heating it with a couple of 
NaOH2 beads but to no success. Thank you very much for your comments.

Regards

Julio





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