[Histonet] PAS-diastase staining

[Roy, Lisa]

I have been using the spit method for years with great results.  Depar slide to water, add saliva directly to slide for 10 minutes, rinse and stain PAS as usual.  Also American MasterTech has diastase malt that works well.
Lisa

-----Original Message-----
From: Rene J Buesa [mailto:rjbuesa at yahoo.com] 
Sent: Friday, May 29, 2015 11:17 AM
To: Julio Benavides; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining

Sigma porcine diastase is very good.A cheaper option is very disgusting but had been done for decades, just spit on the section but that will carry bacteria and, although I have seen doing it, I have never done it.René J.  


     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


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     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples (ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase digestion (I am assuming that PAS afterwards is the normal protocol for PAS) -A commercial source for diastase working in these samples. Is the porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


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Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
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