[Histonet] PAS-diastase staining

[Rene J Buesa]

Sigma porcine diastase is very good.A cheaper option is very disgusting but had been done for decades, just spit on the section but that will carry bacteria and, although I have seen doing it, I have never done it.René J.  


     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples 
(ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase 
digestion (I am assuming that PAS afterwards is the normal protocol for PAS)
-A commercial source for diastase working in these samples. Is the 
porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


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     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples 
(ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase 
digestion (I am assuming that PAS afterwards is the normal protocol for PAS)
-A commercial source for diastase working in these samples. Is the 
porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
     On Friday, May 29, 2015 10:01 AM, Julio Benavides <j.benavides at eae.csic.es> wrote:
   

 Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples 
(ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase 
digestion (I am assuming that PAS afterwards is the normal protocol for PAS)
-A commercial source for diastase working in these samples. Is the 
porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio


_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet