[Histonet] PAS-diastase staining

[Julio Benavides]

Hi there,

I am trying to do a PAA-diastase in formalin-fixed embedded samples 
(ovine liver). I would be most grateful if someone could give me a hand in:

-Protocol (enzyme concentration, time of digestion...) for diastase 
digestion (I am assuming that PAS afterwards is the normal protocol for PAS)
-A commercial source for diastase working in these samples. Is the 
porcine amylase from sigma any good? cheaper options?

As always,

thank you very much for all your comments

Cheers

Julio