[Histonet] IHC breast markers: changing source of NBF

Anne Van Binsbergen annigyg at gmail.com
Sun May 10 21:43:58 CDT 2015


Greetings esteemed Histonetters

We use commercially prepared NBF in pre-filled containers for collection and submission of all tissue. 

We use 'home-made' NBF ( Millonigs as per Freda Carson) in the holding tanks on the gross station and in all our tissue processors. 

We also strictly adhere to CAP regulations re fixation times for all breast tissue (6-72 hrs) - - our preferred lower end cut-off is 8 hrs. 

Recently we have had some variable IHC breast marker staining outcomes. 

Question: Could the use of 2 'different ' sources of NBF affect the staining of predictive markers?

Your expert opinion is highly appreciated

Regards from SandyLands
annieinarabia

Sent from my iPhone


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