[Histonet] Post fixing IHC slides in formalin

[Obregon, Cecilia]

Our facility implemented the post fixing of IHC slides years ago only for tissues like breast, cell blocks, or anything that tends to fall off. We baked the slides in the oven for 30 minutes, and then post fixed them for an additional 30 min in formalin 'fumes'. It works great on breast resections specimens, and it doesn't interfere with staining. 
Thank you,

Cecilia M. Obregon
Memorial Regional Hospital
3501 Johsnon Street
Hollywood, FL 33021

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Barry Rittman [barryrittman <@t> gmail.com]
Sent: Saturday, March 21, 2015 2:20 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Post fixing IHC slides in formalin

There are always discussions about fixation but I have never seen comments
about using vapor fixation for post fixing or for fixing fresh frozen
specimens.
Vapor fixation is simple to use and does not require any solvent as it uses
the tissue fluids in the case of fresh frozen sections or the solution
remaining after IHC.
Especially useful for formaldehyde, alcohol,  acetone, osmium tetroxide etc.
Barry

On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian <thisisann <@t> aol.com> wrote:

> Does anyone post fix their IHC slides in formalin in an effort to try to
> reduce tissue loss?  If so, does anyone have a protocol for this that they
> have used and have seen good results?
>
>
> If you have any other suggestions which can help to reduce tissue loss
> during IHC staining, I would love to hear from you.
> Thanks,
> Ann
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