[Histonet] Tol Blue

[Linda Prasad (SCHN)]

The mast cells are meant to turn purple. It’s a metachromatic stain. So the tol blue stains the tissue blue and the mast cells purple.

Mast cell granules                      	Purple
Acid Mucosubstance                      	Purple
Nuclei                                  Blue

Mast cells are rich in heparin. This allows them to be stained via acid mucopolysaccharide and metachromatic staining techniques. Churukian and Schenk developed this metachromatic dye-technique to reduce the excessive background staining that is common with metachromatic dye techniques.


Linda Prasad | Senior Scientist | Histopathology
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn
Sent: Wednesday, 25 March 2015 9:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Tol Blue

Try the method at:

http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm

Bryan Llewellyn

Kimberly Marshall wrote:
> ?Hello my fellow Histo Techs.  Have a question I just know someone out there can answer for me.
>
> In canine tissue, we are having problems with the Tol Blue for mast cell.  Am experiencing metachromasia, or the mast cells turning purple not blue.  I have attempted to decrease time, or add time, but its not helping.  My pathologist says he has had this issue before.  So question is.  Could it be the mast cell in a dog does not stain the same? Is there another stain that may work?  Any help will be much appreciated.
>
>
> Thanks in Advance.
>
> Kimberly Marshall H.T.(ASCP)
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> Kimberly Marshall H.T.(ASCP)
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