[Histonet] Tol Blue

[Bryan Llewellyn]

Try the method at:

http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm

Bryan Llewellyn

Kimberly Marshall wrote:
> ?Hello my fellow Histo Techs.  Have a question I just know someone out there can answer for me.
>
> In canine tissue, we are having problems with the Tol Blue for mast cell.  Am experiencing metachromasia, or the mast cells turning purple not blue.  I have attempted to decrease time, or add time, but its not helping.  My pathologist says he has had this issue before.  So question is.  Could it be the mast cell in a dog does not stain the same? Is there another stain that may work?  Any help will be much appreciated.
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> Thanks in Advance.
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> Kimberly Marshall H.T.(ASCP)
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> Kimberly Marshall H.T.(ASCP)
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> Histology/Lab Supervisor
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