[Histonet] Removing paraffin sections from glass slides

[Hugh Luk]

Hi Anna,

You could deparaffinize/rehydrate to 10% (or so) bleach.  Section(s) comes right off after ~1-60 minutes (or so).  Did this by accident (that was a bad day).  Deparaffinzation via Rene J Buesa's DWS (http://www.histosearch.com/ADP7HistologyWithoutXylene.pdf) method, or simply quick (20 seconds each) xylene/alcohol/water should suffice.  

I don't know what bleach will do to the charge on your plus slide, but I assume you should "Clean" with acid to be useful(?).

I have been queried about reusing slides from several physicians in the past, but never been organized enough to try it.

Good luck, in whatever you are doing.
Hugh
Hawaii


> ----------------------------------------------------------------------
> Date: Mon, 22 Jun 2015 14:24:50 +0000
> From: "Coffey, Anna (NIH/NCI) [C]" <anna.coffey at nih.gov>
> To: "histonet at lists.utsouthwestern.edu"
> 	<histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Removing paraffin sections from glass slides
> Message-ID: <5C3E10119A1B824FBE92B08279F74A91025973BC at msgb10.nih.gov>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello,
> 
> I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides.
> 
> Thanks!
> Anna
> 
> Anna Coffey, MS, HTL(ASCP)CM
> Histotechnologist
> Center for Advanced Preclinical Research
> Frederick National Laboratory for Cancer Research
> Leidos Biomedical Research, Inc.
> Bld 539, 224
> Frederick, Maryland 21702
> anna.coffey at nih.gov
> 301-846-1730
> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Mon, 22 Jun 2015 14:52:30 +0000
> From: "Weems, Joyce K." <Joyce.Weems at emoryhealthcare.org>
> To: "'Coffey, Anna (NIH/NCI) [C]'" <anna.coffey at nih.gov>,
> 	"histonet at lists.utsouthwestern.edu"
> 	<histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Removing paraffin sections from glass slides
> Message-ID:
> 	<E3A4EBD57A691646BCCED4AA5911A030011C747155 at e14mbx12n.Enterprise.emory.net>
> 	
> Content-Type: text/plain; charset="us-ascii"
> 
> This is what I would do...
> 
> Soak the coverslip off in xylene
> Either  - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry..
> 
> OR   - air dry out of xylene and wipe off with a wet gauze  - then do the alcohol dip
> 
> Whichever worked best. Just my 2 cents..
> 
> Happy Monday!!
> 
> Joyce Weems
> Pathology Manager
> 678-843-7376 Phone
> 678-843-7831 Fax
> joyce.weems at emoryhealthcare.org
> 
> 
> 
> www.saintjosephsatlanta.org
> 5665 Peachtree Dunwoody Road
> Atlanta, GA 30342
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> -----Original Message-----
> From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov]
> Sent: Monday, June 22, 2015 10:25 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Removing paraffin sections from glass slides
> 
> Hello,
> 
> I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides.
> 
> Thanks!
> Anna
> 
> Anna Coffey, MS, HTL(ASCP)CM
> Histotechnologist
> Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc.
> Bld 539, 224
> Frederick, Maryland 21702
> anna.coffey at nih.gov
> 301-846-1730
> 
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> End of Histonet Digest, Vol 139, Issue 22
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