[Histonet] Removing paraffin sections from glass slides

[John Kiernan]

Why shouldn't the slide surfaces remain positively charged after removing the sections? Water, alcohol and xylene are all routinely used, and don't impair electrostatic forces that help to hold the sections on the slide. The positively charged surface is not a coating that can be washed or rubbed off.
Joyce Weems's suggested method did not involve anything that might react chemically with the cationic (amino-hydrocarbon) groups that are covalently bound to the polysilicate structure of the glass. See http://publish.uwo.ca/~jkiernan/adhesivs.htm

John Kiernan
Anatomy & Cell Biology
University of Western Ontario
London, Canada
= = =
On 23/06/15, Jamal  <j.rowaihi at alborglaboratories.com> wrote:
> Dear Colleague
> I just want to remind you..
> 
> After all of that procedure of removing the paraffin sections from the
> positive charged slides.
> The slides will not be positive charged anymore and not suitable for IHC.
> Also the cost of the detergents and the chemicals which you used for
> removing the tissue section is more costly than the slides.
> 
> 
> 
> Best Regards,
> 
> 
> Jamal M. Al Rowaihi	Anatomic Pathology Supervisor | Al Borg
> Medical Laboratories | Mobile +966 503629832|
> j.rowaihi at alborglaboratories.com 
> Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA |
> Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
> www.alborglaboratories.com
> 
> 
> -----Original Message-----
> From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] <Joyce.Weems at emoryhealthcare.org]> 
> Sent: Monday, June 22, 2015 5:53 PM
> To: 'Coffey, Anna (NIH/NCI) [C]'; histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Removing paraffin sections from glass slides
> 
> This is what I would do...
> 
> Soak the coverslip off in xylene
> Either - rehydrate back to water and just wipe the tissue off - then dip
> the slides in absolute and let dry..
> 
> OR - air dry out of xylene and wipe off with a wet gauze - then do the
> alcohol dip
> 
> Whichever worked best. Just my 2 cents..
> 
> Happy Monday!!
> 
> Joyce Weems
> Pathology Manager
> 678-843-7376 Phone
> 678-843-7831 Fax
> joyce.weems at emoryhealthcare.org
> 
> 
> 
> www.saintjosephsatlanta.org
> 5665 Peachtree Dunwoody Road
> Atlanta, GA 30342
> 
> This e-mail, including any attachments is the property of Saint Joseph's
> Hospital and is intended for the sole use of the intended recipient(s). It
> may contain information that is privileged and confidential. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> are not the intended recipient, please delete this message, and reply to the
> sender regarding the error in a separate email.
> 
> 
> -----Original Message-----
> From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov] <anna.coffey at nih.gov]>
> Sent: Monday, June 22, 2015 10:25 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Removing paraffin sections from glass slides
> 
> Hello,
> 
> I'm wondering if anyone has experience removing dried unstained paraffin
> sections from charged glass slides. I don't need to preserve the sections,
> just want to reuse the slides.
> 
> Thanks!
> Anna
> 
> Anna Coffey, MS, HTL(ASCP)CM
> Histotechnologist
> Center for Advanced Preclinical Research Frederick National Laboratory for
> Cancer Research Leidos Biomedical Research, Inc.
> Bld 539, 224
> Frederick, Maryland 21702
> anna.coffey at nih.gov
> 301-846-1730
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ________________________________
> 
> This e-mail message (including any attachments) is for the sole use of
> the intended recipient(s) and may contain confidential and privileged
> information. If the reader of this message is not the intended
> recipient, you are hereby notified that any dissemination, distribution
> or copying of this message (including any attachments) is strictly
> prohibited.
> 
> If you have received this message in error, please contact
> the sender by reply e-mail message and destroy all copies of the
> original message (including attachments).
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
>