[Histonet] Removing paraffin sections from glass slides

[Jamal]

Dear Colleague
I just want to remind you..

After all of that procedure of removing the paraffin sections from the
positive charged slides.
The slides will not be positive charged anymore and not suitable for IHC.
Also the cost of the detergents and the chemicals which you used for
removing the tissue section is more costly than the slides.



Best Regards,


Jamal M. Al Rowaihi	Anatomic Pathology Supervisor       | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowaihi at alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA    |
Phone: +966 12 670 0099	      | Fax: +966 12 676 4984     |
www.alborglaboratories.com


-----Original Message-----
From: Weems, Joyce K. [mailto:Joyce.Weems at emoryhealthcare.org] 
Sent: Monday, June 22, 2015 5:53 PM
To: 'Coffey, Anna (NIH/NCI) [C]'; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Removing paraffin sections from glass slides

This is what I would do...

Soak the coverslip off in xylene
Either  - rehydrate back to water and just wipe the tissue off - then dip
the slides in absolute and let dry..

OR   - air dry out of xylene and wipe off with a wet gauze  - then do the
alcohol dip

Whichever worked best. Just my 2 cents..

Happy Monday!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems at emoryhealthcare.org



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-----Original Message-----
From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov]
Sent: Monday, June 22, 2015 10:25 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Removing paraffin sections from glass slides

Hello,

I'm wondering if anyone has experience removing dried unstained paraffin
sections from charged glass slides. I don't need to preserve the sections,
just want to reuse the slides.

Thanks!
Anna

Anna Coffey, MS, HTL(ASCP)CM
Histotechnologist
Center for Advanced Preclinical Research Frederick National Laboratory for
Cancer Research Leidos Biomedical Research, Inc.
Bld 539, 224
Frederick, Maryland 21702
anna.coffey at nih.gov
301-846-1730

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