[Histonet] Removing paraffin sections from glass slides

[Weems, Joyce K.]

This is what I would do...

Soak the coverslip off in xylene
Either  - rehydrate back to water and just wipe the tissue off - then dip the slides in absolute and let dry..

OR   - air dry out of xylene and wipe off with a wet gauze  - then do the alcohol dip

Whichever worked best. Just my 2 cents..

Happy Monday!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems at emoryhealthcare.org



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-----Original Message-----
From: Coffey, Anna (NIH/NCI) [C] [mailto:anna.coffey at nih.gov]
Sent: Monday, June 22, 2015 10:25 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Removing paraffin sections from glass slides

Hello,

I'm wondering if anyone has experience removing dried unstained paraffin sections from charged glass slides. I don't need to preserve the sections, just want to reuse the slides.

Thanks!
Anna

Anna Coffey, MS, HTL(ASCP)CM
Histotechnologist
Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc.
Bld 539, 224
Frederick, Maryland 21702
anna.coffey at nih.gov
301-846-1730

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