[Histonet] de-waxing protocol on instruments just fact finding

cynthia haynes naje1972 at yahoo.com
Mon Jun 22 21:16:21 CDT 2015

Only if you don't de-wax long enough. 

De-wax for 10 minutes  in xylene  at least 3 changes each. Then dehydrate 5 minutes in 100 %  alcohol 3 changes each. Then 95% alcohol 2 changes each for 5 minutes. Then 80% 2 minutes each and finally 70% 1 minute,then rinse well in DI h2o.

hope this helps. 

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From:"Rachel M Gonzalez" <rachel at gbi-inc.com>
Date:Mon, Jun 22, 2015 at 5:12 PM
Subject:[Histonet] de-waxing protocol on instruments just fact finding


Just beginning to look at instruments DAKO Ventana Leica. We may not have
the budget but if we do.... However talking to the sales reps have been
challenging as everything is proprietary.

This is for research consideration so we will use an open instrument? Which
instrument do you like better open?

Does  manual dewaxing affect the staining? Every rep says yes... but does

What is the time for dewaxing when you start the instrument? What are the
number of steps in the protocol? What is the waste generation like more or
less than manual?

Thanks for your help.

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