[Histonet] Pre-floating tissue sections in dilute alcohol

[Weems, Joyce K.]

I've never done two steps... just pour it in the water bath.. works great.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems at emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-----Original Message-----
From: Gudrun Lang [mailto:gu.lang at gmx.at]
Sent: Sunday, June 07, 2015 6:39 AM
To: 'Garrey Faller'
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol

Some of our histotechs use just cold tapwater for wrinkled sections before transferring them  into the warm waterbath. I don't know, if the added ethanol makes any difference (maybe reducing surface tension).

I would offer the possibility of using the additional waterbath, but let the histotechs decide, if it is necessary to use it.

Gudrun Lang
Leading histotech, Linz Austria


-----Ursprüngliche Nachricht-----
Von: Garrey Faller [mailto:garreyf at gmail.com]
Gesendet: Samstag, 06. Juni 2015 16:29
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol

Hi everyone,

I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf
<http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf>
Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?

Thanks in advance.
Garrey Faller
Pathologist
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