[Histonet] tissue fixation-formaldehyde concentrations

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sat Jun 6 14:08:47 CDT 2015

I have to agree with the student, John.
Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault...   we all are/were there at some point;-)
Sure...I agree with you re the using of the word  Paraformaldehyde as a fixative....
I often "sigh" when used.

However, differential fixation ( zonal fixation) has always been an issue.
We often see the zonal effects of this?
Particularly in lymph nodes ( parenchymatous tissues)
Obviously, when immersion fixing.
Most often ...NOT with agitation.


Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
SE1 1UL 
020 7848 6813    

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